Differences in heat resistance were similar, regardless of the model used and were kept at all temperatures tested. A clear effect of the pH of the heating medium was observed, decreasing the D120 value from pH 7 to pH 3 buffer down to one third. Isothermal survival curves showed shoulders that were accurately characterized by means of both models. Thermal treatments were carried out in pH 3, 5 and 7 McIlvaine buffer and in a courgette soup. The aim of this research was to evaluate the effect of the heating medium characteristics (pH and buffer/food) on the thermal inactivation of B. sporothermodurans spores when exposed to isothermal and non-isothermal heating and cooling treatments and the suitability of non-linear Weibull and Geeraaerd models to predict the survivors of these thermal treatments. In recent years, highly thermo-resistant mesophilic spore-forming bacteria belonging to the species Bacillus sporothermodurans have caused non-sterility problems in industrial sterilization processes. All rights reserved.Įffect of the medium characteristics and the heating and cooling rates on the nonisothermal heat resistance of Bacillus sporothermodurans IC4 spores.Įsteban, MarÃa-Dolores Huertas, Juan-Pablo Fernández, Pablo S Palop, Alfredo Results showed that multiple dynamic heating profiles, when analyzed simultaneously, can be used to accurately estimate the kinetic parameters while significantly reducing estimation errors and data collection. Ordinary least squares one-step regression and sequential procedures were applied for estimating these parameters. The aim of the present work was to study the inactivation kinetics under both static and dynamic conditions in a vegetable soup. They can be used as indicators or surrogates to establish the minimum requirements for heat processes, but it is necessary to understand their thermal inactivation kinetics. High heat-resistant sporeforming bacteria are one of the main causes for spoilage and safety of low-acid foods. ↳ DI 9.One-step global parameter estimation of kinetic inactivation parameters for Bacillus sporothermodurans spores under static and dynamic thermal processes.Ĭattani, F Dolan, K D Oliveira, S D Mishra, D K Ferreira, C A S Periago, P M Aznar, A Fernandez, P S Valdramidis, V Pīacillus sporothermodurans produces highly heat-resistant endospores, that can survive under ultra-high temperature. The files that SporeMaster selects the first time (Spore_Game, Spore_Graphics and Text) are located in the core Spore data folder (well, Text is inside the folder Locale\your-language). I believe, I have to select other package files instead, right? Which ones? Or do I have to open those that I find within the Data folder of the regular, older Spore folder (not GA)? Is GA still using files in the regular Spore folder, too? Does the game crash, if the replacing species has more complexity than the Grox? Or a different size? Or what is the reason?īut there is also a problem: I downloaded and installed SporeMaster 2beta, but cannot find Spore_Game.package and Text.package inside my GA/Data folder. Is there even a way, to replace each Maxis creation with one of my own ones? That would be the dream of all dreams!Īnd I need to know, why replacing the Grox with another species causes the game to crash. I need instructions how to remove all these Maxis creations. That was too fast for me as modding novice (have still no master to teach me the power).
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